Satellite Educational Workshops
The ABRF 2009 Satellite Educational Workshops will be held before the main conference. There will be five workshops. They will cover genomics and proteomics technologies, including next generation sequencing, proteomics instrumentation, HPLC theory and practice, recombinant proteins, and informatics for proteomics. Listed below are the schedules, fees, descriptions, and links to the detailed programs for each of these workshops.
Four of the workshops will be held on Saturday, Feb. 7, 2009, from 8:00 am - 4:00 pm. The laboratory on recombinant proteins will be a two day workshop, held from Friday, Feb. 6, 2009, to Saturday, Feb. 7, 2009, from 8:00 am - 4:00 pm.
Registration to the ABRF 2009 main conference is required for registration to a Satellite Educational Workshop. Satellite Workshop registration is limited to one workshop per meeting attendee. Information about registration fees and workshop descriptions are listed below.
To register, please use the online registration form.
Already registered the meeting, but would like to register for a Satellite Educational Workshop? Then click here for the paper registration form. Registration for a Satellite Educational Workshop cannot be added to an existing Meeting Registration via the online registration form. Please complete the paper registration form for Satellite Educational Workshops only and fax the completed form to the contact included on the form.
Sponsorship opportunites are availabile for each Satellite Educational Workshop. Please visit the Satellite Workshop Sponsorship web page for information on how to become a workshop sponsor.
Satellite Workshop Registration Fees
| Workshop Name | Date/Time | Early Bird Rate (Register by 12/15) |
Advanced Rate (Register by 1/26) |
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(sw1) Next Generation DNA Sequencing |
Saturday, Feb 7 8 am - 4 pm |
$270.00 | $295.00 | $320.00 |
| (sw2) Proteomics Instrumentation | Saturday, Feb 7 8 am - 4 pm |
$270.00 | $295.00 | $320.00 |
|
(sw3) Recombinant Protein Laboratory |
Friday, Feb 6 - Saturday, Feb 7 8 am - 4 pm |
$325.00 | $350.00 | $375.00 |
| (sw4) HPLC Theory and Practice | Saturday, Feb 7 8 am - 4 pm |
$270.00 | $295.00 | $320.00 |
| (sw5) Proteome Informatics | Saturday, Feb 7 8 am - 4 pm |
$270.00 | $295.00 | $320.00 |
Satellite Workshop Descriptions
Click here for the Program for all Satellite Educational Workshops.
(sw1) Next Generation DNA Sequencing
Full Title: Massively Parallel Sequencers in the Core Facility: Applications and Computation
Description: This full day educational workshop will cover the current state of the art of using next generation sequencing technologies in core facilities. The morning session will cover applications, including chemistries, sample preparation and instrument operation for the Roche 454, Illumina GAII, and ABI SOLiD platforms. The afternoon session will cover the hardware and software support necessary for running next generation sequencing platforms. Topics will include required computational infrastructure, data management tools and downstream data analysis software. The workshop will focus on practical information for both current users and potential future users of these platforms.
Course Instructors:
Michael Zianni, Ohio State University (organizer)
Caprice Rosato, Oregon State University (co-organizer)
Michelle Detwiler, Roswell Park Cancer Institute (co-organizer)
Charles Nicolet, University of California at Davis (co-organizer)
Peter Schweitzer, Cornell University (co-organizer)
Alvaro Hernandez, University of Illinois
James Hadfield, Cambridge Research Institute, United Kingdom
Todd Smith, Geospiza
Scott Givan, Oregon State University
Faye Schilkey, National Center for Genome Resources
Jer-Ming Chia, USDA-ARS/CSHL
Click here for the Next Generation DNA Sequencing Workshop Program
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(sw2) Proteomics Instrumentation
Full Title: Introduction to Proteomics Platforms and Mass Spectrometry: Applications for Qualitative and Quantitative Studies.
Description: This full day educational workshop will provide an overview of proteomics analytical tools and will focus on the role of mass spectrometry in proteomics research. It will be aimed at both beginners and intermediate level users and will be useful for graduate students, post-docs, and principle investigators interested in utilizing these powerful technologies. Mass spectrometry platforms that will be covered include ESI and MALDI sources and how they interface with triple-quadrupole, ion trap, time-of-flight, and other mass analyzers. Practical applications on some of the major platforms will be discussed, including LC/MS-based, gel-based, and direct MALDI imaging. Quantitative proteomics approaches will be addressed, including stable-isotope and label-free LC-MS strategies, and Difference Gel Electrophoresis (DIGE/MS) coupled with multivariate statistical analysis.
Course Instructors:
David Friedman, Vanderbilt University (organizer)
Hayes McDonald, Vanderbilt University (co-organizer)
Click here for the Proteomics Instrumentation Workshop Program
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(sw3) Recombinant Protein Laboratory
Full Title: Recombinant Protein Laboratory
Description: This two day educational workshop will provide an opportunity for participants to purify proteins from cell pellets using a variety of resins and perform several analyses to assess purity and yield. Both conventional procedures and an automated system will be used. Participants will also use a novel high-throughput screening approach to assess solubility conditions. Basic theory will be covered during interactive discussion sessions.
Course Instructors:
John Hawes, Miami University (organizer)
Richard Heath, St. Jude Children's Research Hospital (co-organizer)
James Bryson, Bristol-Myers Squibb
Preston Hensley, Pfizer
Cynthia Kinsland, Cornell University
Francis Rajamohan, Pfizer
**Please sign up soon. Registration for this hands on laboratory workshop is limited to a maximum of 12 participants.**
Click here for the Recombinant Protein Laboratory Program
(sw4) HPLC Theory and Practice
Full Title: Practical Aspects of Protein and Peptide HPLC Separations for Proteomics
Description: This full day educational workshop will cover the various modes of liquid chromatography used in the separation of proteins and peptides, and provide examples of how to use these techniques for a wide range of separations in the field of proteomics. Topics will include: (1) chromatography for sample preparation; (2) HPLC for fractionation of complex mixtures in proteomics (including intact proteins and/or protein digests); (3) strategies for multidimensional fractionation of complex mixtures; (4) LCMS of intact proteins (top down proteomics) and LCMS of protein digests (bottom up proteomics); (5) affinity isolation of phosphopeptides, glycopeptides, and peptides with other post-translational modifications; and (6) a survey of modes of chromatography suitable for intact proteins, including ion-exchange, HIC, HILIC, and SEC. Emphasis will be placed on practical tips and techniques to maximize separation, recovery and throughput, while minimizing sample handling problems and variability.
Course Instructors:
Andrew Alpert, PolyLC Inc. (organizer)
Kerry Nugent, Michrom BioResources (co-organizer)
Click here for the HPLC Theory and Practice Workshop Program
(sw5) Proteome Informatics
Full Title: Real-life Proteome Bioinformatics for Laboratories
Description: This workshop will focus on the key issues encountered when setting up or upgrading a practical, efficient informatics workflow in a proteomics laboratory. Proteome informatics is an essential part of proteomics research today, enabling the connection between mass spectra and peptides, and links peptides with proteins. Furthermore, biostatistical analyses have become instrumental in establishing quantitative differences between proteomes from different samples using mass spectrometry. This workshop will give an overview of existing MS and MS/MS search engines, such as SEQUEST, Mascot, X!Tandem and OMSSA, and discuss practical ways of assessing the reliability of the identifications reported by these search engines with tools such as PeptideProphet and decoy database searches for error estimation. The effects and importance of sequence databases such as the NCBI's non-redundant database, UniProt, IPI and Ensembl, will be covered, along with existing protein inference methods such as ProteinProphet. Additional topics will include an overview of key resources and a description of quantitation strategies, related data analysis issues and the corresponding statistics. The workshop will conclude with an exploration of the available strategies to identify mutated sequences and modified proteins, and the standardisation of data for publication and dissemination.
Course Instructors:
Lennart Martens, EMBL-European Bioinformatics Institute, UK (organizer)
David Tabb, Vanderbilt University (co-organizer)
Brian Searle, Proteome Software
Kathryn Lilley, Cambridge University, UK
Click here for the Proteome Informatics Workshop Program